2.24801 BIOCHEMISTRY & MOLECULAR BIOLOGY I

This course is conducted by the Discipline of Biochemistry and Molecular Biology. It covers the main biochemical principles involved in the homeostasis of a functional human body. Introduction to the Functional Groups and Chemical bonds and their importance in Biomolecules; an overview of the major functions of the cellular organelles and subcellular fractions in Eukaryotic cells. Water as an important solvent and the role it plays in controlling the pH and buffer system in the body. Carbohydrates chemistry and tis relationship as a source of energy: the structural and metabolism of carbohydrate is discussed. Synthesis of protein involving the amino acids and polypeptides ion the structure of the protein. Significance of Nucleotides and Nucleic Acids in synthesizing DNA, RNA and the role it plays in the syntheses of proteins. The classification of lipids according to common names, systematic nomenclature and properties of saturated and some common poly-unsaturated fatty acids. Role of the biological membranes in regulation of body components and metabolism including the mechanism of membrane transport. Enzymes as catalysts of many biochemical reactions aided by coenzymes and prosthetic groups as cofactors; structure and factors which may affect the rate of reaction are discussed. The role of Electron Transport Chain; Oxidative Phosphorylation and the formation and detoxification of superoxides.

Aim: To provide students with an understanding of biochemistry and of biochemical concepts as a base for later work in other subjects within the medical sciences.

Contact hours: 6 hours (2 lectures, 1 tutorial, 3 practical).

Assessment: Course work (assignments, tests, practical write-up) 60%, final exam (theory & practical) (40%).

Text:

Murray, R.K., Granner, D.K., Mayes, P.A., Rodwell, V.W., Harper's Biochemistry 24th Ed, Prentice-Hall Int., 1996

Delvin, T. M. (Ed), Textbook of Biochemistry with Clinical Correlations, 4th Ed., 2001, John Wiley & Sons, Sydney.